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EICN procedure for new users

1.      Electron Microscopy
Electron microscopy is a powerful extension of optical techniques into the sub-optical wavelength sized world. Using electrons accelerated to relativistic speeds, these microscopes can image individual atoms.
Transmission electron microscopy requires a thin sample, about a micron or less, and thinner is better. It uses a variety of techniques to image and characterize samples. For example:
·         High resolution imaging can resolve individual atoms.
·         Cryo-EM can reveal structures and molecular interactions of
         biological complexes.
·         STEM imaging can differentiate atom types.
·         X-ray spectroscopy (EDX) can quantify elemental composition.  
·         Tomography can resolve the 3D structure of materials and
         biological complexes.  
 
2.     How can electron microscopy facilitate your research project? 
EICN can assist you in achieving success in your research by providing state-of-the-art transmission electron microscopy instruments and assisted usage services. EICN offers advanced electron imaging techniques for visualizing materials, nanomachines, and cellular structures at atomic or nanometer scales in 2D and 3D. 
 
3.      How does the EICN operate? 
EICN users can either request a staff expert to image their sample or be trained to operate the electron microscopes themselves. Users new to electron microscopy are trained and practice on the CM120, T12, and JEM1200EX. More advanced users are trained to operate the TF20, Titan S/TEM, and Titan Krios. Help with advanced electron microscopy techniques is available upon request. Contact the Technical Director, Xing Zhang or EICN Associate Technical Director, Ivo Atanasov to see how EICN can assist with your project. 
 
4.      Create Core Lab Management System (CLMS) account.
EICN uses the Core Lab Management System (CLMS), a CNSI centralized reservation system, to manage reservations for all the instruments. Create a CLMS account here to have access to the system. The CLMS account is subject to approval from CNSI and EICN. Normally, it takes a few days to process a new account request. For further information on CLMS accounts, click here. EICN is not responsible for CLMS functionality, so please address your inquiries and problems directly to CLMS administrators:
Jeff Korerat jkorerat@cnsi.ucla.edu  (For problems involving funding and/or Recharge IDs)
Maryam Ariannejad MArian@mednet.ucla.edu (For technical issues involving CLMS)
 
5.     Complete EICN Application form.
Read policy, sign the EICN application, User Agreement, and the EHS safety Agreement (right-click to download a combined PDF file. This form must be filled out, signed by the user and PI, and presented to EICN personnel prior to the training session. Please indicate if you are planning to use any dangerous materials at EICN. User is responsible for providing all permits required by UCLA Environmental Health & Safety in relation to the project the user wants to conduct at EICN. Copies of valid permits should accompany the application form.
 
6.     Obtain training at EICN.
Training is required in order to use EICN equipment. Thereafter, users are permitted to use the facility 24 hours a day, 7 days a week. Please email eicnhelp@cnsi.ucla.edu to set an appointment for training. We guarantee training within three weeks from your request date. As of July 1, 2011, all training sessions are billed using the hourly rates associated with each instrument. Training is not available without a completed EICN Application form.
 
7.       Users are permitted to use the EICN facility after creating a CLMS account, presenting a signed EICN User Agreement and completing training. Using instruments without a reservation on CLMS is not permitted.                
Users must comply with general CNSI and internal EICN policies and be aware of charges related to using EICN. 
Users have equal access to the EICN facility on a first come, first serve basis during normal working hours via CLMS.
 
8.       For grant reporting and application purposes, all publications reporting data generated from the EICN instruments must contain the following statement at the end of the publication's acknowledgement section:   (1) For use of all instruments other than Titan Krios:     "The authors acknowledge the use of instruments at the Electron Imaging Center for NanoMachines supported by NIH (1S10RR23057 to ZHZ) and CNSI at UCLA."     (2)  For use of Titan Krios:     "The authors acknowledge the use of instruments at the Electron Imaging Center for NanoMachines supported by NIH (1S10RR23057 and 1S10OD018111), NSF (DBI-1338135) and CNSI at UCLA."     (3)  For West/Midwest Consortium:     "The authors acknowledge the use of instruments at the Electron Imaging Center for NanoMachines supported by NIH (1S10RR23057, 1S10OD018111 and 1U24GM116792), NSF (DBI-1338135) and CNSI at UCLA."  

 

We look forward to hearing from you!

EICN personnel.
 
AttachmentSize
UA Standard Operating Procedure_07_06_2010.pdf18.28 KB
EICN-application-2017.pdf145.96 KB
Submitted by Xiaorui on Fri, 05/30/2008 - 21:01.